Equipment Model

Leica MATEO FL

Description

The Leica MATEO FL is an inverted fluorescence microscope designed for imaging of cells and tissues, supporting fluorescence-based assays. Funding Agency: Department of Biotechnology (DBT).

Lab

MSLS - CAIF

Institute

Manipal School of Life Sciences (MSLS)

Location

Manipal School of Life Sciences, Eshwar Nagar, Manipal, Udupi, Karnataka 576104, India

• Applications

  • Immunofluorescence Imaging - Visualization of fluorescently labeled proteins and subcellular structures.

  • Colocalization Analysis - Spatial overlap assessment of two or more fluorescent markers to study protein interactions.

  • Fluorescence Intensity Quantification - Signal-based measurement of protein expression levels across cellular compartments.

  • AI-Assisted Cell Counting and Confluency Estimation - Automated quantification of cell number and culture confluency using inbuilt AI algorithms.

• Key Features

  • Optimized for cell culture imaging.

  • Objectives: 10x, 20x, and 40x.

  • Fluorescence channels: Blue and Green

  • Blue channel: Excitation: 395/25, Emission: 460/50

  • Green channel: Excitation: 470/40, Emission: 525/40

  • Suitable for fixed and live cell imaging.

• Limitations

  • Not a confocal system; no optical sectioning or z-stack capability.

  • Limited to two fluorescence channels.

  • Oil immersion objectives are not supported.

  • Image quality may vary across objective magnifications.