Equipment Model

ThermoFisher Scientific NanoDrop 2000

Description

The NanoDrop 2000 is a microvolume UV-Vis spectrophotometer designed for the quantification and analysis of nucleic acids, proteins, and other biomolecules.

Lab

MIV

Institute

Manipal Institute of Virology (MIV)

Location

Manipal Institute of Virology, Madhav Nagar, MAHE, Manipal, Udupi, Karnataka 576104, India

• Applications

  • Quantification of nucleic acids (DNA, RNA).

  • Protein concentration analysis using UV-Vis spectroscopy.

  • Purity assessment of biomolecules based on absorbance ratios.

  • Microvolume sample analysis with minimal sample loss.

  • Used in molecular biology, genomics, and proteomics research.

• Key Features

  • Requires only 1-2 µL of sample for analysis.

  • No cuvettes or capillaries needed, reducing sample waste.

  • Fast measurement time—results in seconds.

  • Wide absorbance range (190-840 nm) for diverse applications.

  • Pre-programmed nucleic acid and protein quantification methods.

• Limitations

  • Limited to microvolume sample analysis (not suitable for large volumes).

  • Requires proper calibration and maintenance for accuracy.

  • Not suitable for highly turbid or particulate-containing samples.