Equipment Model

ThermoFisher Scientific 3500 XL Genetic Analyzer

Description

The Applied Biosystems 3500 XL Genetic Analyzer is an 8-capillary sequencing instrument engineered for high-precision Sanger sequencing applications.

Lab

MIV

Institute

Manipal Institute of Virology (MIV)

Location

Manipal Institute of Virology, Madhav Nagar, MAHE, Manipal, Udupi, Karnataka 576104, India

• Applications

  • Small genome sequencing (maximum of up to 1000 bp per run).

  • Targeted sequencing of specific genomic regions.

  • Mutation detection and variant analysis.

  • Forensic DNA analysis and STR genotyping.

  • Clinical and research applications in genetic disease studies.

• Key Features

  • 8-capillary electrophoresis system for high-throughput sequencing.

  • Enhanced process control for validated laboratory environments.

  • Supports fluorescent dye-based detection for high accuracy.

  • Optimized for Sanger sequencing with high read length capability.

  • Compatible with multiple primer strategies for whole-genome sequencing.

• Limitations

  • Maximum read length of 1000 bp per sequencing run.

  • Whole-genome sequencing requires multiple overlapping primers.

  • Throughput is limited compared to next-generation sequencing (NGS) platforms.

  • Sample quality and preparation significantly affect sequencing accuracy.