Equipment Model

Sysmex Partec CyFlow Cube 6

Description

The Flow Cytometry facility enables the analysis and sorting of cells based on their properties for molecular and functional studies.

Lab

MSLS - CAIF

Institute

Manipal School of Life Sciences (MSLS)

Location

Manipal School of Life Sciences, Eshwar Nagar, Manipal, Udupi, Karnataka 576104, India

• Applications

  • Isolation and characterization of specific cell types.

  • Quantification of absolute cell numbers and viability.

  • Immunophenotyping for identifying surface and intracellular markers.

  • Stem cell enumeration and classification.

  • Cell cycle analysis and ploidy determination.

  • Apoptosis and necrosis detection.

  • Mitochondrial function analysis and metabolic profiling.

• Key Features

  • High-throughput cell analysis and sorting capabilities.

  • Multi-parameter detection for detailed cellular characterization.

  • Quantitative and qualitative assessment of cell populations.

  • Applicable for research in immunology, oncology, and stem cell biology.

• Limitations

  • Requires fluorescently labeled antibodies and dyes for detection.

  • Expertise is needed for data interpretation and gating strategies.

  • Limited sensitivity for very low-abundance cell populations.